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- ДокументSCIENTIFIC SUBSTANTIATION OF TECHNOLOGY OF PASTA BASED ON FRESHWATER MUSSELS ENRICHED WITH SELENIUM(2020) Mykola Holovko, Tetyana Holovko, Vladyslav Prymenko, Anna Helikh, M. ZherebkinThe possibility of using dietary selenium-protein additive "Neoselen" for enrichment of pastes based on mussels with organic selenium has been investigated. The formulation of the pastes involved the use of the soft body of the freshwater mmussels of the genus Anodonta. The influence of the introduction of different percentage of the additive "Neoselen" on the organoleptic properties of the developed pastes based on freshwater mussels was investigated. It is found that the addition of additive "Neoselen" even more than 5% does not impair the taste and color of the developed pastes. Moisture content, moisture retention, fat-holding capacity and active acidity of the developed pastes were investigated. The moisture-holding and fat-holding capacity of the developed paste is determined. The difference in the consistency of pastes based on freshwater mussels with different percentage of unpowered additives was investigated by determining the values of plastic viscosity and modulus of elasticity. The regularities of the influence of the additive "Neoselen" on the general chemical and mineral composition of pastes based on freshwater mussels have been established. It is established that the addition of 1%, 3% or 5% in the paste of additive "Neoselen" can enrich it by 7.8, 23.4 and 39 mkg of selenium, respectively. Studies have shown that in the composition of both the control and experimental samples of pastes in the amount of protein, minerals and water content. With the increase of the content of the additive "Neoselen" in the formulation of pastes, the content of protein and minerals increases. The complex of qualitative characteristics of the developed pastes is investigated. The regularities of the influence of the component composition on the organoleptic and physicochemical and functional-technological characteristics of the minced systems based on freshwater mussels and the additive "Neoselen" have been established. It is established that the optimal doses of the use of additive "Neoselen" in the formulation of pastes ranges from 3-5%.
- ДокументFEATURES OF ORGANOCHLORINE PESTICIDES RESIDUES ACCUMULATION IN MELLIFEROUS PLANTS, BEE POLLEN AND HONEY(2020) N. Kasianchuk, O. Berhilevych, I. Negay, L. Dimitrijevich, T. MarenkovaHoney is natural product, which is produced by honeybees and due to contain of minerals, vitamins, simple sugars, organic acids, antioxidants and enzymes, it is considered as product with good nutritional and therapeutic properties. All beneficial characteristics of honey are reduced if it is contaminated with extraneous chemicals include pesticides. The purpose of this research was to study pesticides residues accumulation in the flowers of different types of melliferous plants, bee pollen and honey obtained from Odessa Region during 2015–2017 years. A total of 121 samples of flowers of melliferous plants and trees, 78 samples of bee pollen and 104 samples of honey were investigated with gas-liquid chromatography method. We found that all investigated samples were positive to such organochlorine pesticides as α, β, γ–isomers of hexachlorocyclohexane and dichlorodiphenyltrichloromethylmethane and its derivatives in different concentration. Our studies determined that high level of organochlorine pesticides was in samples of sunflower flowers whith average concentrations of α, β, γ–isomers of hexachlorocyclohexane 7.51±0.04 μg/kg, and DDT 6.98±0.02 μg/kg. Flowers of buckwheat and others field herbs (including as wild herbs as fodder crops) had even less concentration of these pesticides. Besides, the obtained results show that in the flowers of fruit trees (cherry, apple, pear, cherry, peach, cherry plum) had less concentration of studied pesticides in compere to forest trees (white acacia, linden). The results of bee pollen and honey samples examination have shown the same situation. The highest concentration of α, β, γ–isomers of hexachlorocyclohexane and dichlorodiphenyltrichloromethylmethane was in sample from sunflower origine with average consentration 3.52±0.05 and 3.77±0.03 μg/kg in bee pollen, 2.74±0.01 and 2.53±0.03μg/kg in honey respectively. However, the detected concentrations of pesticide residues in all samples were lower than the maxim admissibile limits, according to national and EU standards. Despite this, we suggest that regularly monitoring of pesticide residues in melliferous plants and apiculture products at national level need to grantee of quality and safety of honey and protection of consumers` health.
- ДокументOBTAINING AND CHARACTERISTIC OF MUROPEPTIDES OF PROBIOTIC CULTURES CELL WALLS(2020) Антоніна Капустян, N. ChernoРозглянуто можливість отримання муропептидів пептидогліканів клітинних стінок Lactobacillus delbrueckii subsp. Bulgaricus B-3964 шляхом комбінації застосування автолітичних процесів та ферментативної обробки біомаси за участю лізоциму та папаїну. Встановлено, що найбільш значні автолітичні зміни біомаси мають місце при застосуванні високотемпературної обробки (90°С протягом 30 хв) на завершальній стадії логарифмічної фази росту бактерій. Так, на восьмій годині інкубації при 37°С вміст амінокислот у культуральному середовищі складає 1,8 мг/см3, а при 90°С – 5,7 мг/см3. Із метою подальшої деструкції автолізату біомаси та отримання низькомолекулярних фрагментів пептидоглікану, досліджували процес його ферментолізу лізоцимом та папаїном окремо та при їхній комбінації. Найвищий вміст НМП у реакційному середовищі мав місце при ферментолізі біомаси Lactobacillus delbrueckii subsp. Bulgaricus B-3964 композицією ферментів при співвідношенні лізоцим:папаїн 1:2. При концентрації ферментів 10 мг/см3 вміст НМП складав 7,2 мг/см3 на 8-му годину інкубації реакційної суміші. Результати досліджень показали, що ефективність ферментолізу автолізату значно вища. Так, кількість НМП у ферментолізаті, який отримали при обробці автолізату композицією лізоцим:папаїн 1:2 при концентрації ферментів 10мг/см3 та тривалості процесу протягом 8 годин на 36% більша, ніж за аналогічних параметрів без застосування процесу автолізу. Методом гель-хроматографії доведено, що у складі ферментолізату присутні фракції білкових сполук з молекулярною масою в межах 70–90 кДа, 30–40 кДа та 294–650 Дa. Молекулярна маса останньої фракції відповідає масі мурамилдипептиду.
- ДокументAN INNOVATIVE TECHNOLOGY OF WAFFLES WITH FUNCTIONAL PROPERTIES(2020) Е. Iorgacheva, G. Korkach, T. Lebedenko, O. KotuzakiThe high-priority tasks of today’s confectionery industry include the development and introduction of functional-purpose confections. These products are supposed to be of high technological qualities, be effective in production to the extent possible, and have physiological functional properties due to the physiologically valuable ingredients in their composition. These ingredients include the synbiotic complex composed of probiotics (bifidobacteria, lactobacilli) and prebiotics (inulin and lactulose). This complex has been studied in this research. The paper gives reasons why waffles with fatty fillings are the ones to which the synbiotic complex should be added. It has been established what mass fraction of the ingredients at what stage of fatty filling production should be added. It has been studied how the synbiotic complex affects the structural and mechanical properties of the fatty filling: its effective viscosity, adhesive strength, and critical shear stress. It has been found that the maximum shear rate that still allows obtaining a high-quality filling is 5.4 s-1. The specific pull-off force of the fatty filling with the synbiotic added becomes lower compared to that of the control sample. This helps reduce the energy consumed in the course of moulding waffles. An innovative manufacturing technology for waffles with synbiotic supplements has been developed and proved practical. It includes the stages of preparing microencapsulated microorganisms and adding prebiotics, and provides the technological parameters of fatty filling preparation. It has been established that the synbiotic included into the composition of the new fatty filling decreases the maturation of waffle blocks by 40–45%, and allows reducing the layer of filling to 1–1.5 mm. It has been proven that the synbiotic complex as an ingredient in the fatty filling of waffles will allow developing and manufacturing confections that improve human microflora. These new specialised products will be socially significant, as they will help solve a topical and important task of the confectionery industry: prevent gastrointestinal diseases.
- ДокументCHOKEBERRY POWDER AS AN IMPROVER FOR PASTA(2020) Z. Koshak, A. PokrashinskayaIn the course of the study, the effect of dosages of black chokeberry powder on the quality of pasta made from flour with different gluten content was studied. The studies used normal-quality flour and low-gluten flour. To reduce the gluten content in wheat flour, it was mixed with potato starch. As a result of “dilution” of gluten with starch, its content decreased from 25 to 2.5 %. The resulting mixture was used to obtain pasta with the introduction of different amounts of black chokeberry powder. The effect of different doses of chokeberry powder on the gluten content of the obtained mixtures was determined by planning experiment 22 “with a star” in the StatGraphicsPlus package. It has been established that with increasing dosages of chokeberry powder, there is a decrease in gluten content and an increase in the content of pectin substances. In the finished pasta, the mechanical strength and the amount of dry matter transferred to the cooking water were determined. According to the data obtained, a chart has been developed to determine the dosage of chokeberry powder and the amount of starch introduced, at which the quality of pasta that meets the requirements of the standard is achieved. Thus, the introduction of 5.0 % aronia chokeberry powder with a gluten content in flour of about 18 % will make it possible to obtain products with a strength of 0.66 N and the amount of solids transferred to cooking water of less than 7.9 %. When using flour of normal quality, the addition of chokeberry powder in an amount of 5 % has a positive effect on the mechanical strength of pasta, increasing it by 20 % compared to the control sample. Other quality indicators, including cooking properties, are within the permissible norm. Production tests were conducted at Lidahleboproduct OJSC. Black chokeberry powder was added in an amount of 5%, pasta was made on Buhler production line with a capacity of up to 2000 kg per shift. According to the results of the tests, TU BY 500134647.012-2018 "Aronia" pasta and TI BY 500134647.001-2019 "Technological instruction for the production of pasta with Aronia" food additive were developed.