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    Evaluation of soil microbial community parameters in correlation with metal pollution gradient
    (2021) Kolomiets, V.; Parvanova, O.
    This report provides an information about specific physicochemical site description, focusing on determining the microbial content of the sampled lead and copper polluted soil of the site location, its quality. It contains reasoning for the analysis, methodological part with bibliographic studies of the location given. Available on–site bacteria in the top soil layer of 0 – 0.5m tested for the microbiological content analysis, using latest Next Generation Sequencing (NGS) technologies – “Illumina MISEQ” sequencer with read length of 250 cycles both ways on a 457 base pare DNA amplicon. Since microorganisms are sensitive to low pollution concentration levels, this implies, that they could serve as more accurate pollution indicators. Thus, five boreholes were made to sample the soil at the research site, which has been located as a quay between the city and meadow landscape. Almost each soil sample has different metal concentration. According to the NGS results Acidobacteria (up to 26%), Proteobacteria (up to 20%), Actinobacteria (up to 15%) and Firmicutes (up to 12%) bacteria phyla are dominant in the sampled soil. Also found Nitrospirae bacteria, which is a globally distributed group of nitrite oxidizers, playing important role in nitrogen cycle. Comparing the results of this analysis and the review made in the annex 1 – a few similar genera were found. One of the reasons to the fact, that the most abundant phylum in given soil is Acidobacteria – is that this phylum was also observed to be found in metal–contaminated soils and sediments according to Thrash J. and Coates J., 2015, however in current situation the same correlation has not beenfound. Next steps for the project: AMR (anti metal resistance) analysis, binding the correlation between the concentration of the pollutant and its impact on the microbial population.